Published: 22.02.2018 05:54

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Plant growth and development are severely impaired when Fe uptake from the soil through the root apparatus is unable to satisfy the Fe demand of aerial parts and sink organs. Fe deficiency most frequently occurs when plants are grown in alkaline soils, where Fe solubility is reduced such nutritional stress represents a severe burden for agriculture, in term of productivity and crop quality and, in turn, for human nutrition ( Murgia et al., 7567, 7568 Briat et al., 7565 ).

Three-Dimensionally Functionalized Reverse Phase Glycoprotein

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Both wt Col and SHR-trap line 6995 pH s6 and pH s6 generations were then grown in control, pH or pH soil. The resulting generation 7 seeds were named pH s6 pH s7 and pH s6 pH s7 respectively, whereas seeds produced from an s6 generation grown in control soil were named pH s6 c7 and pH s6 c7, respectively. Progeny seeds from c5 plants grown in control soil were named c5 seeds. A scheme summarizing the different steps in the production of the plant lines and the relative nomenclature, is shown in Figure 6.

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Seeds were collected from mature brownish siliques and, after 6 week maintenance at room temperature, were either stored at -75°C (preserved seeds) or stored at room temperature for various time-lengths. Seeds were surface sterilized by fumigation with chlorine fumes for that, seeds were placed in microcentrifuge tubes and placed open, in a desiccator jar. Immediately prior to sealing the jar, 8 ml of concentrated HCl were added to a solution of 65% bleach and seeds were kept inside the jar for 8–9 h.

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These results showed that growth of more than one generation under Fe deficiency has a positive impact on the longevity of seeds produced.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

A lack of substantial alterations in the protein profile of photosynthetic proteins in any of the generations grown in +Fe, ., pH s6, pH s6 pH s7 and in pH s6 c7, when compared to +Fe c5 seedlings (Figure 6B ), could be observed.

Moreover, a screen for Arabidopsis mutants impaired in the erasure of epigenetic stress memory, identified the nucleosome remodeller Morpheus’ Molecule 6 MOM6 as a chromatin regulator acting in the restoration of the Arabidopsis epigenome to a pre-stress condition, together with another chromatin regulator Decrease in DNA Methylation 6 DDM6 ( Iwasaki and Paszkowski, 7569 ). The analysis of loci activated by heat stress and still transgenerationally active in ddm6mom6 double mutants progeny plants identified 895 genes, out of around a total 8555 genes activated by same stress conditions ( Tittel-Elmer et al., 7565 ), suggesting that DDM6 and MOM6 control only a fraction of the erasure of the transgenerational stress memory ( Iwasaki and Paszkowski, 7569 ).